High-performance Electrical Variable Resistor Sensor for E.coli O157:H7 Detection

Authors

  • R. D. A. A. Rajapaksha Institute of Nano Electronic Engineering, Universiti Malaysia Perlis, 01000 Kangar, Perlis, Malaysia.
  • U. Hashim Institute of Nano Electronic Engineering, Universiti Malaysia Perlis, 01000 Kangar, Perlis, Malaysia. School of Microelectronic Electronic Engineering, Universiti Malaysia Perlis, 01000 Kangar, Perlis, Malaysia.
  • M. N. Afnan Uda School of Microelectronic Electronic Engineering, Universiti Malaysia Perlis, 01000 Kangar, Perlis, Malaysia.
  • C. A. N. Fernando Nano Technology Research Laboratory, Department of Electronics, Faculty of Applied Sciences, Wayamba University of Sri Lanka, Kuliyapitiya, Sri Lanka.

Keywords:

DNA Sensor, E.coli O157, H7, Hybridization, Interdigitated Electrode, Variable Resister Sensor,

Abstract

Nowadays interdigitated electrode (IDE) based sensors have stimulated increasing interest in the application of biosensor field. A large number of finger electrodes as comb structure gain high sensitivity through electrical measurements. In this research study, we have demonstrated a novel mechanism as biocontrollable variable resistor through solid state conducting Polymer Bridge to detect single-stranded E. coliO157:H7 DNA. The gap of AuIDE sensor on Si substrate was used to create DNA biosensor. Functionalization steps of the AuIDE to create biosensor was based on silanization by APTES, immobilization of E.coliO157:H7 synthetic probe singlestranded DNA (ssDNA), blocking with tween-20. The well fabricated AuIDE biosensor was physically characterized by using scanning electron microscope (SEM) and high power microscope (HPM). Molecular assembly of the functionalized biosensor was analyzed structurally using Energy-dispersive Xray spectroscopy (EDX) and electrically using current–voltage measurements (I-V). The selectivity of the biosensor was identified electrically using complementary, noncomplementary and single base mismatch ssDNA targets. Blocking step with tween-20 was important to detect target specifically. The obtained variations in current indicate the varied concentrations of E. coli targets and it is confirmed that biosensor is suitable to detect different concentrations in the range from 10 fM to 10 µM.

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Published

2018-05-31

How to Cite

Rajapaksha, R. D. A. A., Hashim, U., Afnan Uda, M. N., & Fernando, C. A. N. (2018). High-performance Electrical Variable Resistor Sensor for E.coli O157:H7 Detection. Journal of Telecommunication, Electronic and Computer Engineering (JTEC), 10(1-17), 61–64. Retrieved from https://jtec.utem.edu.my/jtec/article/view/4167

Issue

Vol. 10 No. 1-17: Recent Trends in Biomedical Engineering Systems and Technologies II

Section

Articles

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